Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection.
Identifieur interne : 000548 ( Main/Exploration ); précédent : 000547; suivant : 000549Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection.
Auteurs : Mingyang He [République populaire de Chine] ; Yan Xu ; Jiangling Cao ; Ziguo Zhu ; Yuntong Jiao ; Yuejin Wang ; Xin Guan ; Yazhou Yang ; Weirong Xu ; Zhenfang FuSource :
- Protoplasma [ 1615-6102 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Protéines végétales, Vitis.
- métabolisme : Fractions subcellulaires, Protéines végétales, Vitis.
- parasitologie : Maladies des plantes, Vitis.
- Interactions hôte-pathogène, Peronospora.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Plant Proteins.
- genetics : Vitis.
- metabolism : Subcellular Fractions, Vitis.
- parasitology : Plant Diseases, Vitis.
- Host-Pathogen Interactions, Peronospora.
Abstract
Downy mildew, caused by the oomycete Plasmopara viticola, is a serious fungal disease in the cultivated European grapevines (Vitis vinifera L.). The class 10 of pathogenesis-related (PR) genes in grapevine leaves was reported to be accumulated at mRNA level in response to P. viticola infection. To elucidate the functional roles of PR10 genes during plant-pathogen interactions, a PR10 gene from a fungal-resistant accession of Chinese wild Vitis pseudoreticulata (designated VpPR10.2) was isolated and showed high homology to PR10.2 from susceptible V. vinifera (designated VvPR10.2). Comparative analysis displayed that there were significant differences in the patterns of gene expression between the PR10 genes from the two host species. VpPR10.2 was induced with high level in leaves infected by P. viticola, while VvPR10.2 showed a low response to this inoculation. Recombinant VpPR10.2 protein showed DNase activity against host genomic DNA and RNase activity against yeast total RNA in vitro. Meanwhile, recombinant VpPR10.2 protein inhibited the growth of tobacco fungus Alternaria alternata and over-expression of VpPR10.2 in susceptible V. vinifera enhanced the host resistance to P. viticola. The results from subcellular localization analysis showed that VpPR10.2 proteins were distributed dynamically inside or outside of host cell. Moreover, they were found in haustorium of P. viticola and nucleus of host cell which was associated with a nucleus collapse at 10 days post-inoculation. Taken together, these results suggested that VpPR10.2 might play an important role in host plant defense against P. viticola infection.
DOI: 10.1007/s00709-012-0384-8
PubMed: 22327469
Affiliations:
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Yangling, Shaanxi 712100, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100</wicri:regionArea><wicri:noRegion>Shaanxi 712100</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Yan" sort="Xu, Yan" uniqKey="Xu Y" first="Yan" last="Xu">Yan Xu</name></author><author><name sortKey="Cao, Jiangling" sort="Cao, Jiangling" uniqKey="Cao J" first="Jiangling" last="Cao">Jiangling Cao</name></author><author><name sortKey="Zhu, Ziguo" sort="Zhu, Ziguo" uniqKey="Zhu Z" first="Ziguo" last="Zhu">Ziguo Zhu</name></author><author><name sortKey="Jiao, Yuntong" sort="Jiao, Yuntong" uniqKey="Jiao Y" first="Yuntong" last="Jiao">Yuntong Jiao</name></author><author><name sortKey="Wang, Yuejin" sort="Wang, Yuejin" uniqKey="Wang Y" first="Yuejin" last="Wang">Yuejin Wang</name></author><author><name sortKey="Guan, Xin" sort="Guan, Xin" uniqKey="Guan X" first="Xin" last="Guan">Xin Guan</name></author><author><name sortKey="Yang, Yazhou" sort="Yang, Yazhou" uniqKey="Yang Y" first="Yazhou" last="Yang">Yazhou Yang</name></author><author><name sortKey="Xu, Weirong" sort="Xu, Weirong" uniqKey="Xu W" first="Weirong" last="Xu">Weirong Xu</name></author><author><name sortKey="Fu, Zhenfang" sort="Fu, Zhenfang" uniqKey="Fu Z" first="Zhenfang" last="Fu">Zhenfang Fu</name></author></analytic><series><title level="j">Protoplasma</title><idno type="eISSN">1615-6102</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Host-Pathogen Interactions (MeSH)</term><term>Peronospora (MeSH)</term><term>Plant Diseases (parasitology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Subcellular Fractions (metabolism)</term><term>Vitis (genetics)</term><term>Vitis (metabolism)</term><term>Vitis (parasitology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fractions subcellulaires (métabolisme)</term><term>Interactions hôte-pathogène (MeSH)</term><term>Maladies des plantes (parasitologie)</term><term>Peronospora (MeSH)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Vitis (génétique)</term><term>Vitis (métabolisme)</term><term>Vitis (parasitologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines végétales</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Subcellular Fractions</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Fractions subcellulaires</term><term>Protéines végétales</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term><term>Vitis</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Vitis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Host-Pathogen Interactions</term><term>Peronospora</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Interactions hôte-pathogène</term><term>Peronospora</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Downy mildew, caused by the oomycete Plasmopara viticola, is a serious fungal disease in the cultivated European grapevines (Vitis vinifera L.). The class 10 of pathogenesis-related (PR) genes in grapevine leaves was reported to be accumulated at mRNA level in response to P. viticola infection. To elucidate the functional roles of PR10 genes during plant-pathogen interactions, a PR10 gene from a fungal-resistant accession of Chinese wild Vitis pseudoreticulata (designated VpPR10.2) was isolated and showed high homology to PR10.2 from susceptible V. vinifera (designated VvPR10.2). Comparative analysis displayed that there were significant differences in the patterns of gene expression between the PR10 genes from the two host species. VpPR10.2 was induced with high level in leaves infected by P. viticola, while VvPR10.2 showed a low response to this inoculation. Recombinant VpPR10.2 protein showed DNase activity against host genomic DNA and RNase activity against yeast total RNA in vitro. Meanwhile, recombinant VpPR10.2 protein inhibited the growth of tobacco fungus Alternaria alternata and over-expression of VpPR10.2 in susceptible V. vinifera enhanced the host resistance to P. viticola. The results from subcellular localization analysis showed that VpPR10.2 proteins were distributed dynamically inside or outside of host cell. Moreover, they were found in haustorium of P. viticola and nucleus of host cell which was associated with a nucleus collapse at 10 days post-inoculation. Taken together, these results suggested that VpPR10.2 might play an important role in host plant defense against P. viticola infection.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22327469</PMID><DateCompleted><Year>2014</Year><Month>01</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1615-6102</ISSN><JournalIssue CitedMedium="Internet"><Volume>250</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Protoplasma</Title><ISOAbbreviation>Protoplasma</ISOAbbreviation></Journal><ArticleTitle>Subcellular localization and functional analyses of a PR10 protein gene from Vitis pseudoreticulata in response to Plasmopara viticola infection.</ArticleTitle><Pagination><MedlinePgn>129-40</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00709-012-0384-8</ELocationID><Abstract><AbstractText>Downy mildew, caused by the oomycete Plasmopara viticola, is a serious fungal disease in the cultivated European grapevines (Vitis vinifera L.). The class 10 of pathogenesis-related (PR) genes in grapevine leaves was reported to be accumulated at mRNA level in response to P. viticola infection. To elucidate the functional roles of PR10 genes during plant-pathogen interactions, a PR10 gene from a fungal-resistant accession of Chinese wild Vitis pseudoreticulata (designated VpPR10.2) was isolated and showed high homology to PR10.2 from susceptible V. vinifera (designated VvPR10.2). Comparative analysis displayed that there were significant differences in the patterns of gene expression between the PR10 genes from the two host species. VpPR10.2 was induced with high level in leaves infected by P. viticola, while VvPR10.2 showed a low response to this inoculation. Recombinant VpPR10.2 protein showed DNase activity against host genomic DNA and RNase activity against yeast total RNA in vitro. Meanwhile, recombinant VpPR10.2 protein inhibited the growth of tobacco fungus Alternaria alternata and over-expression of VpPR10.2 in susceptible V. vinifera enhanced the host resistance to P. viticola. The results from subcellular localization analysis showed that VpPR10.2 proteins were distributed dynamically inside or outside of host cell. Moreover, they were found in haustorium of P. viticola and nucleus of host cell which was associated with a nucleus collapse at 10 days post-inoculation. Taken together, these results suggested that VpPR10.2 might play an important role in host plant defense against P. viticola infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>He</LastName><ForeName>Mingyang</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Jiangling</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Ziguo</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Jiao</LastName><ForeName>Yuntong</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yuejin</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Guan</LastName><ForeName>Xin</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yazhou</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Weirong</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Fu</LastName><ForeName>Zhenfang</ForeName><Initials>Z</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>02</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Austria</Country><MedlineTA>Protoplasma</MedlineTA><NlmUniqueID>9806853</NlmUniqueID><ISSNLinking>0033-183X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044742" MajorTopicYN="Y">Peronospora</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013347" MajorTopicYN="N">Subcellular Fractions</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2011</Year><Month>11</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>01</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>2</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>2</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>1</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22327469</ArticleId><ArticleId IdType="doi">10.1007/s00709-012-0384-8</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Physiol. 1996 Oct;112(2):727-33</Citation><ArticleIdList><ArticleId IdType="pubmed">8883384</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2010 Aug 20;10:184</Citation><ArticleIdList><ArticleId IdType="pubmed">20727162</ArticleId></ArticleIdList></Reference><Reference><Citation>J Plant Physiol. 2010 Jan 15;167(2):121-30</Citation><ArticleIdList><ArticleId 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Jiangling" sort="Cao, Jiangling" uniqKey="Cao J" first="Jiangling" last="Cao">Jiangling Cao</name><name sortKey="Fu, Zhenfang" sort="Fu, Zhenfang" uniqKey="Fu Z" first="Zhenfang" last="Fu">Zhenfang Fu</name><name sortKey="Guan, Xin" sort="Guan, Xin" uniqKey="Guan X" first="Xin" last="Guan">Xin Guan</name><name sortKey="Jiao, Yuntong" sort="Jiao, Yuntong" uniqKey="Jiao Y" first="Yuntong" last="Jiao">Yuntong Jiao</name><name sortKey="Wang, Yuejin" sort="Wang, Yuejin" uniqKey="Wang Y" first="Yuejin" last="Wang">Yuejin Wang</name><name sortKey="Xu, Weirong" sort="Xu, Weirong" uniqKey="Xu W" first="Weirong" last="Xu">Weirong Xu</name><name sortKey="Xu, Yan" sort="Xu, Yan" uniqKey="Xu Y" first="Yan" last="Xu">Yan Xu</name><name sortKey="Yang, Yazhou" sort="Yang, Yazhou" uniqKey="Yang Y" first="Yazhou" last="Yang">Yazhou Yang</name><name sortKey="Zhu, Ziguo" sort="Zhu, Ziguo" uniqKey="Zhu Z" first="Ziguo" last="Zhu">Ziguo Zhu</name></noCountry><country name="République populaire de 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